Studi In Vitro Biakan Sel Endotel Kelinci pada Media Cair dan Semi Padat  

Oleh:

Harlina, Eva ; Riadi, Yudi ; Priosoeryanto, Bambang Pontjo

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 10 no. 1 (Mar. 2003), page 34-37.
Topik: In Vitro; Sel Endotel Kelinci; Arteri Femoralis
Fulltext: Eva Harlina, Bambang Pontjo Priosoeryanto, Yudi Riadi-Bernard.pdf (54.7KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.5 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Endothelial cells derived from femoralis artery of rabbit were succesfully cultured in vitro in liquid medium of Dulbeco modified essential medium-Ham’s F-12 (DME/F-12) and semi solid medium of collagen gel. The cells cultured in liquid medium grew and formed colony with a pattern of monolayer sheet. They were round to oval and spindle in shape. The cells were grown in collagen gel by using floating and gel-embedded system to show their three-dimensional structure. The growth pattern of the cells cultured in both collagen gel systems showed a formation of irregular fibrous-like structure, elongated cells and colony with duct-like structure as well as radiating projection pattern and formation of a large lumen like structure. Histological findings using Haematoxylin and Eosin staining showed development of a compact cell colony which formed a monolayer sheet with a cell layer of fibroblast and a thin collagen layer. Immunohistochemically, endothelial cells showed positive reaction for anti-Factor VIII Related-Ag (F VIII R-Ag), with smooth fiber pattern in cytoplasm running along the cell border.

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