Antibacterial Property of A Coral-Associated Bacterium Pseudoalteromonas Luteoviolacea Against Shrimp Pathogenic Vibrio Harveyi (In Vitro Study)  

Oleh:

Sabdono, Agus ; Martens, Torben ; Bachtiar, Tonny ; Grossart, Hans-Peter ; Radjasa, Ocky Karna ; Simon, Meinhard

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 12 no. 2 (Jun. 2005), page 77-81.
Topik: antibacterial; Vibrio Harveyi; Bacteria; PCR
Fulltext: 44.pdf (54.98KB)

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Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.7 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

A coral-associated bacterium was succesfully screened for secondary metabolities production based on PCR amplification of the nonribosomal peptide synthetase gene and was identified as closely related to Pseudoalteromonas Iuteoviolacea based on its 16S rDNA.The bacterium was found to inhibit the growth of shrimp pathogenic bacterium tested, Vibrio harveyi.To characterize the inhibiting metabolite, a 279 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for peptide synthetases and revealed a high similarity to NosD (40% identity), a multifunctional peptide synthetase from Nostoc sp. GSV224,and Ndab (44% identity), a peptide synthetase module of Nodularia spumigena.

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