In Vitro Fertilization and Embryo Development of Vitrified Ovine Oocytes Stressed in Sucrose  

Oleh:

Sukra, Yuhara ; Toelihere, Mozes R. ; Supriatna, Iman ; Djuwita, Ita ; Boediono, Arief ; Priyono, Srihadi Agung

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 12 no. 2 (Jun. 2005), page 73-76.
Topik: EMBRYONIC FERTILIZATION; Fertilization; Embryo
Fulltext: 43.pdf (84.74KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.7 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Experiments were conducted on the morphology, fertilization and embryo development rate of vitrified ovine oocytes matured in vitro. Three vitrification solutions were used for vitrificition.PBS supplemented with 1% BSA, 30% ethylene glycol was added by one of three different sucrose concentrations, 1.00 M (VS1), 0.50 M (VS2), and 0.25 M (VS3).The results showed that the percentages of normal vitrified oocytes after warming were 78 and 63% in VS1 and VS2, respectively, which was significantly higher as compared with VS3 (35%). Zygote viability after 18 h was 57, 43, and 40%, for VS1, VS2, VS3, respectively which was not significantly different. The incidence of polyspermic penetration increased with increasing sucrosed concentration, i. e. 23, 11, and 9% in VS1, VS2, VS3, respectively as compared with unvitrified oocytes (4%).The cleavage rate of vitrified oocytes in VS1 was 13,2% which was significantly lower (p<0,05) compared to those of unvitrified control oocytes (70.00%). Hence, a high sucrose concentration is a beneficial for maintaining the oocyte structure during the processes of vitrification and thawing, which ultimately results in increased in vitro fertilitation rates.

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