Kloning DNA Genom Pengapit Transposon dari Mutan Nonpatogenik Xanthomonas Axonopodis pv Glycines M715  

Oleh:

Tjahjono, Budi ; Rusmana, Alma Akhdiya ; Suwanto, Antonius

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 12 no. 2 (Jun. 2005), page 57-60.
Topik: kloning; Kloning DNA; Mutan; Xanthomonas Axonopodis S. Rolfsii
Fulltext: 40.pdf (104.71KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.7 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The objective of this work is to clone flanking DNA derived from T n-5 mutagenesis of wild type strain Xanthomonas axonopodis pv.glycines as first step to clone and to identify the gene involved in pathogenicity mechanism. We have localized the flanking DNA fragment from a nonpathogenic mutant of Xag M715. Southern hybridization analysis using 2.8 kb EcoRI from pYR103 as a probe showed that the fragment is located within2.0 kb PstI fragment. A 0,7 kb flanking DNA was amplified using inverse PCR technique, and inserted into pGEM - T Easy vector generating a 3,7 kb recombinant plasmid (pAA01).Southern hybridization analysis of the wild type (YR32) with pAA01 as a probe indicated a hybridization signal located at approxiamately 3.0 kb PstI fragment. DNA sequence analysis revealed that the DNA fragment has a 64% identity to a vir gene of Bacillus anthracis.

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