Construction and Expression of Single Recombinant Peptide Surfactant for Enhanced Oil Recovery (EOR) Application  

Oleh:

Sari, Cut Nanda ; Usman ; Rohmat, Riesa Kw ; Herlina, Leni ; Suliandari, Ken Sawitri ; Kristiawan, Onie ; Dwiyantari, M.M. Sri ; Kristianti, Tati ; Suhandono, Sony

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 11 no. 1 (Mar. 2017), page 28-34.
Topik: enhanced oil recovery; overlapped PCR method; surfactant peptide
Fulltext: 558-1331-1-PB_Ros.pdf (962.7KB)

Isi artikel

Surfactant is generally synthetic chemical, which is effective and reliable. However, the chemicals usually cannot be degraded easily in the environment and could cause damage to the environment. The other possible alternative to produce surfactant is using genetic engineering in order to produce peptide based surfactant. In this research, peptide surfactant was produced using a gene construct which was created using overlapped polymerase chain reaction method (OE-PCR). SDS-PAGE analysis showed that single surfactant peptide construction can be expressed by induction of IPTG 1 mM and after at least twice sonication. This research proved that both two constructions have been successfully expressed by producing peptide in expected size (approximately 15 kDa).

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