Effect of Tempeh Supplementation on the Profiles of Human Intestinal Immune System and Gut Microbiota  

Oleh:

Stephanie ; Ratih, Nine Kirana ; Soka, Susan ; Suwanto, Antonius

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 11 no. 1 (Mar. 2017), page 11-17.
Topik: Akkermansia muciniphila; ELISA; IgA; RT-PCR; tempe
Fulltext: 499-1308-1-PB_Ros.pdf (487.0KB)

Isi artikel

Tempeh is a traditional fermented soybean product from Indonesia. Although tempeh is consumed as daily menu in Indonesia, its nutrigenomic study employing human has not been reported yet. On the other hand, our study in mice showed that tempeh could enhance immune system, especially by increasing secretory immunoglobulin A production in ileum and colon. Tempeh was also found to be potential in modulating composition of gut microbiota. Therefore, the objective of this study was to analyze the impact of tempeh supplementation on the profiles of human intestinal immune system and gut microbiota analysis. A total of 16 participants, comprising of each 8 healthy females and males, aged between 20 and 23 were recruited to this study. The volunteers consumed 200 mL milk from day 1-8 followed by consumption of 100 grams steamed tempeh each day from day 9-24. Fecal samples taken on day 9 and 25, were analyzed with half sandwich ELISA for IgA enumeration. In addition, fecal samples collected on day 0, 9, and 25, were analyzed for total Akkermansia muciniphila employing quantitative real time PCR. The result of this study suggested that tempeh supplementation might act as paraprobiotic and slimming agents since tempeh enhanced production of IgA and increased population of A. muciniphila in human intestinal tracts.

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