Evaluation of Rapid Detection Kit against Avian Influenza A Virus and H5 Subtype for Field Sample  

Oleh:

Wibowo, Michael Haryadi ; Untari, Tri ; Artanto, Sidna ; Putri, Krisdiana ; Amanu, Surya ; Asmara, Widya

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 21 no. 1 (2016), page 48–55.
Topik: Avian influenza; rapid detection kit; specificity and sensitivity
Fulltext: 26792-56463-2-PB_Ros.pdf (826.25KB)

Isi artikel

Avian influenza virus is a poultry viral disease, which causes high economic losses. Various efforts have been made to control the disease. One effort required fast and accurate screening diagnostic test. This study aimed to determine the potential of a rapid test kit, namely AIV/H5 Anigen Rapid Test for the detection of AI virus types A and subtype H5 in the fields. Some tests were carried out, e.g. the potential test, crossreaction test, sensitivity and specificity test. The potential test was done to evaluate detection limits of the kit, by having the test of a serial dilution of AI virus positive control. Crossreaction test was done to detect antigens other than AI virus H5N1, e.g. IB virus of Massachusetts strain, IBV strain 491, Newcastle Disease virus, and Escherichia coli. Sensitivity and specificity test were applied to the field samples which clinically and laboratory were confirmed as H5N1 positive. To confirm the result of rapid test was then being done by reverse transcriptase polymerase chain reaction. Based on these results it can be concluded that Anigen Kit AIV/H5 Ag Rapid Test can detect antigencontaining samples having AI virus HA titer up to 26 of type A virus, and up to 25 for subtype H5 virus. Anigen Kit AIV/H5 Ag Rapid Test showed no crossreactions with Infectious Bronchitis virus, Newcastle Disease virus, and Escherichia coli. Anigen A Rapid Test Kit AIV Ag showed a sensitivity of 50% and specificity of 100%, while Anigen Ag Rapid Test Kit AIV/H5 showed a sensitivity of 25% and specificity of 100%.

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