Identification of Dengue Virus Serotypes at the Dr. Soetomo Hospital Surabaya in 2016 and Its Correlation with NS1 Antigen Detection  

Oleh:

Akualing, Jeine Stela ; Aryati ; Wardhani, Puspa ; Hadi, Usman

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 23 no. 02 (Mar. 2017), page 151-156.
Topik: Dengue Viral Infection; Dengue Virus Serotypes; NS1 Antigen
Fulltext: I01 v23 n2 p151 kelik2017.pdf (507.29KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Circulating dengue virus serotypes are still changing and differ between regions. Serotype displacement as well as genotype are affecting the occurrence of dengue outbreak. Serotype difference is suspected to be correlated with NS1 detection, but there is still lack of evidence to support this.A cross-sectional study was conducted in February-August 2016 on 60 dengue viral infection (DVI) and 25 non-DVI subjects. Dengue virus RNA was examined in all subjects using Simplexa Dengue Real-Time RT-PCR as well as serotype identification and NS1 using rapid test NS1 Panbio. Difference of proportion between categorical variables was analyzed using Fisher Exact Test. Correlation between serotype and NS1 detection was analyzed by Chi-Square. Dengue virus RNA was detected in 43 of 60 DVI subjects (71.7%). The majority serotype was DENV-3 (62.8%). Serotype displacement has been occurring in Surabaya, from DENV-2 to DENV-1 in the past, and now DENV-3, possibly due to host mobility, viral transport, and geographical factors. NS1 sensitivity was 75% and specificity 100%. NS1 detection between serotypes was significantly different (p=0.002). NS1 detection in DENV-1 was significantly lower than DENV-2 (p=0.007) or DENV-3 (p=0.003). Dengue virus serotype correlated with NS1 detection (p=0.005). Characteristics of serotype and genotype of dengue virus probably affected the secretion of NS1. Serotype displacement has occurred in DVI patients in Surabaya, thus continuous surveillance is needed in order to predict dengue outbreak. Serotypes correlate with NS1 detection. One of the causes concerning false negative results of NS1 is the serotype difference.

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