Detection of Mycobacterium Tuberculosis with TB Antigen Rapid Test in Pulmonary Tuberculosis Patients with Four Types of Spuctum Sample Preparation  

Oleh:

Ilmiah, Miftahul ; Rejeki, Putri Sri ; Tambunan, Betty Agustina

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 23 no. 02 (Mar. 2017), page 119-125.
Topik: TB Ag Rapid Test Device; M.Tuberculosis; Sputum; Regions of Difference
Fulltext: I01 v23 n2 p119 kelik2017.pdf (410.89KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Direct microscopic examination to diagnose TB has many limitations. TB Ag Rapid Test Device is an ICT examination method detecting TB-specific antigen secreted from the regions of difference (RD) of M.tuberculosis. Previous research indicated that tests can not be always performed well. Samples were 30 positive sputum smears by direct microscopy sputum. Sputum samples underwent 4 treatments: routine treatment according to the kit procedure; addition of 0.5 mL 2.5% NALC and whipped according to the kit procedure; vortex treatment followed by centrifugation 10,000g temperature 4°C; addition of 0.5 mL 2.5% NALC, followed by vortexcentrifugation 10,000g temperature 4°C. The results of the antigen examination using TB Ag Rapid Test Device were as follows first routine treatment is 43.3% positive, second treatment had a high positive result (96.7%), third and fourth treatment had positive result 36.7% and 86.7%. Examination of Ag using vortex-centrifugation with a routine examination showed a compatibility of 86.6% (33.3% positive and 53.5% negative) with a Kappa value of 0.724 (p<0.0001). Treatment 2 (addition of 0.5 mL 2.5% NALC) with treatment 4 (addition of 0.5 mL 2.5% NALC- vortex-centrifugation) demonstrated a compatibility of 90% (86.7% positive and 3.3% negative) with a Kappa value of 0.366 (p=0.010). The addition of 0.5 mL 2.5% NALC pretreatment can be used to increase the positive outcome of TB Ag Rapid Test Device for diagnosing pulmonary tuberculosis.

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