Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis  

Oleh:

Adilistya, Tika ; Astrawinata, Dalima A. W. ; Nasir, Ujainah Z.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 48 no. 01 (Jan. 2016), page 41-47.
Topik: Interferon-? Release Assay; ELISPOT; Pleural Tuberculosis
Fulltext: A02 v48 n1 p41 kelik2017.pdf (304.59KB)

Isi artikel

Aim: to evaluate the diagnostic value of an interferon-gamma release assay (IGRA) with enzyme-linked immunospot (ELISPOT) method, T-SPOT.TB, in the diagnosis of pleural TB using pleural fluid mononuclear cells (PFMC). Methods: forty-eight subjects, presumed to have pleural TB with exudative pleural effusion by Light’s criteria, dominated by mononuclear cells, had their pleural fluid specimen tested with T-SPOT. TB, Mycobacterial Growth Indicator Tube (MGIT) culture, and adenosine deaminase (ADA) activity. Other causes of pleural effusion such as heart failure, renal failure, hepatic cirrhosis, and malignancy were excluded. Results: the sensitivity, specificity, positive predictive value, and negative predictive value of the IGRA ELISPOT assay using PFMC for the diagnosis of pleural TB were 100%, 88.89%, 97.5%, and 100%, respectively. Conclusion: IGRA with ELISPOT method performed on PFMC is useful for a rapid and reliable diagnosis of pleural TB in clinical practice, especially in area with high TB burden.

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