Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex  

Oleh:

Wahyuni, Sri ; Susanti, R. ; Iswari, Retno Sri

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 20 no. 2 (Dec. 2015), page 161-166.
Topik: Ficus septica Burm F; ficin enzyme; temperature; pH
Fulltext: 24200-48094-1-SM.pdf (543.22KB)

Isi artikel

This research aims to isolate and characterize the ficin enzyme from Ficus septica stem latex. Ficin from Ficus septica stem latex was isolated using column chromatography. Then enzyme activity was tested at different temperature (40oC, 50oC, 60oC, 70oC) and pH (6.0, 7.0, 8.0) levels. Ficin enzyme activity of joint treatment with variations in temperature and pH was analyzed using two-way ANOVA with a factorial pattern followed by Least Significant Difference (LSD) test. The results showed that temperature treatment significantly affects enzyme activity. However, the treatment of pH and the interaction between temperature and pH did not significantly affect the ficin enzyme activity. There was no significant difference in ficin activity at the incubation temperatures of 40oC and 50oC, as well as 60oC and 70oC. However, comparing the incubation temperatures of 40oC and 50oC with treatment 60°C and 70°C showed a significant difference in ficin enzyme activity. In the treatment of incubation at pH 6, 7 and 8 for ficin enzyme activity showed no significant difference. We concluded that the Ficus septica plant latex contained ficin enzyme with an optimum temperature of 60°C and optimum pH of 6, 7, and 8.

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