Comparison of sdLDL-C Analysis Using Srisawasdi Method and Homogeneous Enzymatic Assay Method on Hypertriglyceridemia Condition  

Oleh:

Nugraha, Gilang ; Edijanto, Soebagijo Poegoeh ; Rianto, Edhi

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 23 no. 01 (Nov. 2016), page 74-79.
Topik: sdLDL-C; Srisawasdi’s Formula; Correlation; Accuracy
Fulltext: I01 v23 n1 p74 kelik2017.pdf (326.74KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Small dense low-density lipoprotein (sdLDL) is the fraction of the smallest particle of low density lipoprotein (LDL) which has a diameter of =25.5 nm. SdLDL particles are highly atherogenic lipoprotein and have even been reported to increase the risk of Coronary Heart Disease (CHD) up to three times. Unfortunately, sdLDL measurements are usually made by complex instruments and intricate techniques, thus making them less suitable to be applied in everyday clinical practice. In 2011, Srisawasdi et al developed a measurement technique to estimate sdLDL-cholesterol (sdLDL-C) using an equation by counting routine lipid profile. It was reported that the increase in the concentration of triglycerides (TG) can lower the estimated correlation of sdLDL-C proposed by Srisawasdi. A decline in correlations can affect the accuracy, resulting in decreased quality of laboratory tests. Therefore, this research aimed to evaluate the examination of sdLDL-C using Srisawasdi’s formula on hypertriglyceridemia condition. Eighty-eight samples were taken to measure Total Cholesterol (TC), TG, High Density Lipoprotein-Cholesetrol (HDL-C) and direct low density lipoproteincholesetrol (dLDL-C) at Dr. Soetomo Hospital. Meanwhile, measurement of sdLDL-C using homogeneous enzymatic assay method was performed at Parahita Laboratory in Dharmawangsa. Results of the analysis show that there was no difference between sdLDL-C using Srisawasdi’s formula and sdLDL-C using homogeneous enzymatic assay method (P=0.000). A decline in correlation found in from the TG concentration group of <100 mg/dL to the TG concentration group of 200-299 mg/dL. The difference correlation value in each group, however, did not affect the accuracy of sdLDL examination using Srisawasdi’s formula (P=0.720) in up to the TG concentration group of <400 mg/dL, with a bias in the whole sample of 34.15%. There are also some limitations of sdLDL-C using Srisawasdi‘s formula. For instance, it can only be used on a TG concentration of less than 200 mg/dL with well-controlled intralaboratorium quality assurance. Thus, further researches need to be conducted to determine the normal value of sdLDL-C using Srisawasdi’s formula.

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