Comparative Ratio of BCR-ABL Genes With PCR Method Using the Codification of G6PD and ABL Genes in Chronic Myeloid Leukemia Patients  

Oleh:

Panjaitan, Tonggo Gerdina ; Prihatni, Delita

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 23 no. 01 (Nov. 2016), page 61-66.
Topik: CML; BCR-ABL Gene; RQ-PCR; G6PD; ABL
Fulltext: I01 v23 n1 p61 kelik2017.pdf (175.37KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Chronic myeloid leukemia was the first malignant disease of hematopoietic that had the relation to genetic lesion. Chronic myeloid leukemia characterized as chronic myeloproliferative disorder, caused by reciprocal translocation of chromosome 9 and 22 which also called the Philadelphia chromosome. Philadelphia chromosome gene form is also called BCR-ABL. Molecular assay in CML is to know mRNA BCR-ABL gene transcript activitythat used for the diagnostic and therapy monitoring of CML patients. Recently, the World Health Organization had published nine standard genes which widely used. The aim of this study is to know the ratio of BCR-ABL/G6PD gene and BCR-ABL/ABL gene in Philadelphia chromosome by comparing. This study included 79 archived biological materials from leukocyte inhabited by CML patients Ph (+) who came to RSUP Dr. Hasan Sadikin Hospital Bandung, which obtained from April 2012-April 2014. The ratio of BCR-ABL/G6PD gene have been evaluated with Real-time Quantification PCR (Ligh tCycler ®) Roche. By using G6PD standard gene could detect b2a2, b3a2, and e1a2 type. The BCR-ABL/ABL gene ratio has been evaluated using (Bioneer®) equipment. Abelson standard gene could only detect the b2a2, b3a2 type, which is more stable than the G6PD gene. The research carried out is cross sectional study with comparative analytic design. The statistical analysis was performed using Wilcoxon non-parametric test. The ratio of mRNA BCR-ABL/G6PD gene and BCR-ABL/ABL gene: [1.93% (0.0–59.7 fg) vs 15.37% (0.04–35.7 copies), p<0.001]. The BCR-ABL gene was undetectable in three (3) samples using ABL gene standard. Based obn this study, it can be concluded that there is significant difference between BCR-ABL/G6PD gene ratio and BCR-ABL/ABL gene one. The same standard gene should be used in subsequent BCR-ABL gene ratio assay for diagnosing and treatment response monitoring.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali