Percentage of CD3+ T Lymphocytes Expressing IFN-y After CFP-10 Stimulation  

Oleh:

Indrasari, Yulia Nadar ; Tambunan, Betty Agustina ; Nugraha, Jusak ; Oetami, Fransiska Sri

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 23 no. 01 (Nov. 2016), page 31-35.
Topik: CD3+; IFN-?; New Cases of Pulmonary TB; Flow Cytometry; CFP-10
Fulltext: I01 v23 n1 p31 kelik2017.pdf (242.96KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis. Cytokine IFN-? production is the main effector mechanism of T lymphocytes. The development of an effective vaccine against TB infections has to consider the factors regulating the production of IFN-?. CFP-10 is an antigen secreted by Mycobacterium tuberculosis, known as a component of a potential vaccine for TB. The purpose of this study was to compare the cellular immune response that is the percentage of CD3+ T-lymphocytes that expresses IFN-? after stimulation of CFP-10 antigen in patients with new cases of pulmonary TB, latent TB, and healthy people. This study used a true experimental design in the laboratory in vitro in cultured PBMC of patients with 8 new cases of pulmonary TB, 7 latent TB and 7 healthy people in the Pulmonary Hospital Surabaya. Examination of the percentage of CD3+ T-lymphocytes expressing IFN-? was done by Flow cytometry (BD FACSCalibur). Results were analyzed by Kruskal-Wallis and one way ANOVA. The mean percentage of CD3+ T-lymphocytes expressing IFN-? in new cases of pulmonary TB after stimulation of CFP-10 antigen (4.36%) was higher than before stimulation (3.50%) (P value=0.015). The mean percentage of CD3+ T-lymphocytes expressing IFN-? in latent TB after CFP-10 antigen stimulation (3.96%) was higher than before stimulation (2.50%) but was not significant (P value=0.367). The mean percentage of CD3+ T-lymphocytes expressing IFN-? in healthy people after stimulation (1.66%) was lower than before stimulation (2.89%) but not significant (P value=0.199). Changes in the percentage of CD3+ T-lymphocytes expressing IFN-? after stimulation of CFP-10 antigen were not significantly different between groups (P value=0.143). Based on this study, it could be concluded that there were an increase in the percentage of CD3+ T-lymphocytes expressing IFN-? in new cases of pulmonary TB after stimulation of CFP-10 antigen. This showed that CD3+ T-lymphocytes expressing IFN-? played a role in protection against pulmonary tuberculosis infection.

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