The Activity of Radical Scavenging of 2,2-Diphenyl-1- Pycrilhydrazil (DPPH) by Ethanolic Extracts of Mengkudu Leaves (Morinda citrifolia L.), Brotowali Stem ( Tinospora crispa L.), Its Water Fraction and Its Hydrolized Fraction  

Oleh:

Irianti, Tatang ; Puspitasari, Andayana ; Machwiyyah L. ; Rabbani H. R.

Jenis: Article from Journal - ilmiah internasional - tidak terdaftar di DIKTI
Dalam koleksi: Majalah Obat Tradisional vol. 20 no. 03 (Sep. 2015), page 140-148.
Topik: Radical Scavenging; Morinda citrifolia L.; Tinospora crispa; L.; Hydrolyzed Water Fraction; DPPH
Fulltext: M01 v20 n3 p140 kelik2017.pdf (959.88KB)

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Perpustakaan FK Nomor Panggil: M01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

We have investigated the antiradical activity of ethanolic extract of mengkudu leaves (Morinda citrifolia L.) and brotowali stem (Tinospora crispa L.) using radical scavenging assay or DPPH radical. Thin layer chromatograms were also studied to estimate the group of compounds that have antiradical activity. The objective of this research was to compare the DPPH antiradical efficiency values of water fraction and acid hydrolyzed water fraction in both extracts. The radical scavenging IC50 value of ethanolic extract, water fraction, and hydrolised water fraction (one and thee hours) of mengkudu leaves were 75,65 µg/mL ; 98,68 µg/mL ; 36,27 µg/mL and 33,36 µg/mL respectively. The IC50 value for brotowali stem were 33.75 µg/mL, 52.29 µg/mL, 31.12 µg/mL and 18.26 µg/mL. The highest antiradical activity was the hydrolyzed water fraction three hour namely 200 µg/mL of this fraction was able to inhibit 33.12% and 18.26 µg/mL DPPH radicals.

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