Antiproliferative Activity of Recombinant Human Interferon Alpha2b on Estrogen Positive Human Breast Cancer MCF-7 Cell Line  

Oleh:

Ningrum, Ratih Asmana ; Wisnuwardhani, Popi Hadi ; Santoso, Adi ; Herawati, Neng

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Pharmacy vol. 26 no. 02 (Apr. 2015), page 86-93.
Topik: Human Interferon Alpha 2b; Pichia Pastoris; Antiproliferative; MCF-7
Fulltext: I03 v26 n2 p86 kelik2017.pdf (847.81KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I03.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Indonesia is the top three countries with hepatitis and moreover has 40.000 cancer mortalities per year. Interferon alpha 2b (IFNa-2b) is a therapeutic standard for cancer and hepatitis B/C treatments. We developed recombinant human interferon alpha 2b (rhIFNa-2b) in methilotropic yeast Pichia pastoris X-33. The protein was produced as extracellular protein with 24 kDa in size. This research was aimed to characterize the protein based on its amino acid sequence and to study its antiproliferative activity on MCF-7 cell line. Amino acid sequencing by using MALDI TOF TOF mass spectrometry with trypsin as proteolytic enzyme identified protein as hIFNa-2b with 33% of amino acid coverage. The antiproliferative activity was determined by 3-[4.5-dimethyltiazol-2il]-2.5-diphenylltetrazolium bromide (MTT) assay. Based on several studies about the synergistic activity of rhIFNa-2b with other anticancer drugs, we combined our rhIFNa-2b with tamoxifen (tmx). The growth percentage of the cells after being treated with 1µM of tmx at various concentrations of rhIFNa-2b was compared with that of untreated cell. This study showed that the antiproliferative activity was dose-dependently. Cell viability assay with calcein and ethidium bromide-based staining by fluorescence microscope confirmed that the rhIFNa-2b had ability to inhibit proliferation of human breast cancer cell line MCF-7.

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