Increasing Sensitivity of MCF-7/Dox Cells Towards Doxorubicin by Hesperetin Through Suppression of P-Glycoprotein Expression  

Oleh:

Sarmoko ; Putri, Dyaningtyas Dewi Pamungkas ; Susidarti, Ratna Asmah ; Nugroho, Agung Endro ; Meiyanto, Edy

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Pharmacy vol. 25 no. 02 (Apr. 2014), page 84-90.
Topik: Breast Cancer; Doxorubicin; Resistance; Hesperetin; P-Glycoprotein
Fulltext: I03 v25 n2 p84 kelik2017.pdf (849.97KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I03.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Long-term use of doxorubicin causes cancer resistance due to overexpression of P-glycoprotein (P-gp), a protein that plays a role in cell drug efflux. The purpose of this study is to determine the action of hesperetin in increasing the cytotoxicity of doxorubicin on MCF-7 cancer cells resistant to doxorubicin (MCF-7/DOX) through suppression of P-gp expression. Cytotoxic assay of single and combinational treatment of doxorubicin and hesperetin were performed by using MTT assay. Apoptosis evidence was examined by using double staining with acridine orange and ethidium bromide dyes, while Pgp expression was determined by using immunocytochemistry. Hesperetin reduced cell viability in dose dependent manner. Both MCF-7 ori and MCF-7/DOX cells gave different responses to hesperetin with the IC50 values of >500µM and 267µM, respectively. Combining treatment of hesperetin and doxorubicin to MCF-7/DOX cells at the dose of 95µM and 230nM increased apoptosis evidence and suppressed P-gp expression. These results suggest that hesperetin enhances the anticancer effect of doxorubicin to resistance MCF-7 cells through suppression of P-gp expression.

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