Anti-HIV dan Subtipe HIV pada Pasien Hemodialisis  

Oleh:

Handajani, Retno ; Thaha, Mochammad ; Amin, Mochamad ; Wungu, Citrawati Dyah Kencono ; Rianto, Edhi ; Pranawa

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 22 no. 02 (Mar. 2016), page 182-186.
Topik: Hemodialysis; Anti-HIV; Subtype HIV; Hemodialisis; Subtipe HIV
Fulltext: I01 v22 n2 p182 kelik2017.pdf (198.93KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Anti-Human Immunodeficiency Virus (Anti-HIV) was performed from 100 plasma Chronic Kidney Disease (CKD) stage 5 patients with continuous hemodialysis (HD) at the Hemodialysis Instalation Dr Soetomo hospital, Surabaya, Indonesia, using three (3) kind of reagents: Tri-line HIV Rapid test Device from Acon for HIV 1/2/O as strips form, Foresight HIV 1/2/O Antibody EIA Test Kit from Acon and Anti-HIV 1+2/Subtype O ELISA from Axiom. HIV RNA and HIV subtype were detected by Reverse Transcription Polymerase Chain Reaction (RT-PCR) based on HIV gag region and analysis of DNA result. Seventy three % patients were hemodialysed twice in a week and only 14% with duration more than five (5) years. Most of the patients (43%) were hemodialysed between 100-300 times. From the 100 plasma samples was obtained only one (1%) man patient plasma sample with positive anti-HIV. A weak positive of RT-PCR result was not succeed to be sequenced for determining the HIV subtype. This cause was suspected due to low levels of HIV RNA in blood. The results of this study was expected can be used as an additional management consideration of hemodialysis patients at the Hemodialysis Unit.

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