Two New Automated, Compared with Two Enzyme-Linked Immunosorbent, Antimullerian Hormone Assays  

Oleh:

Nelson, Scott M. ; Pastuszek, Ewa ; Kloss, Grzegorz ; Malinowska, Iwona ; Liss, Joanna ; Lukaszuk, Aron ; Plociennik, Lukasz ; Lukaszuk, Krzysztof

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 104 no. 04 (Oct. 2015), page 1016–1021.
Topik: Antimullerian Hormone; Assay; Antral Follicle Count
Fulltext: F02 v104 n4 p1016 kelik2016.pdf (744.98KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To compare new automated antimüllerian hormone (AMH) assay performance characteristics from the new automated Elecsys AMH (Roche; Elecsys) and Access AMH (Beckman Coulter; Access) assays with the existing AMH Gen II ELISA (enzyme-linked immunosorbent assay; Gen II; Beckman Coulter) and AMH ELISA (Ansh Labs) assays. Design: Prospective assay evaluation. Setting: University-affiliated clinical chemistry laboratory. Patient(s): Patients referred for serum AMH measurement (n = 83) before start of in vitro fertilization cycle between September 2014 and October 2014. Intervention(s): None. Main Outcome Measure(s): Serum AMH concentration. Result(s): Intra-assay coefficients of variation were low; Ansh = 9.0%; Gen II = 5.8%; Access = 10.7%; and Elecsys = 2.8%. The Passing-Bablok regression equations (pmol/L) were y (Access) = 0.128 + (0.781 × Gen II); and y (Access) = 0.302 + (0.742 x Ansh). For y (Elecys) = 0.087 + (0.729 x Gen II) and y (Elecys) = 0.253 + (0.688 x Ansh Labs). For y (Elecys) = 0.943 - (0.037 × Access). For all the assays, AMH exhibited a moderate positive correlation with AFC (r = 0.62–0.64); number of cumulus oocyte complexes (r = 0.60–0.64); and metaphase II oocytes (r = 0.48–0.50). Accuracy of pregnancy prediction, as determined by area under the receiver operating characteristic curve, was uniformly low for all assays (0.62–0.63). Conclusion(s): The novel automated assays exhibit strong concordance in calibration, but derived values are substantially lower than those obtained from pre-existing assays, with assay-specific interpretation required for routine clinical use. These results highlight the need for an international standard of measurement of AMH.

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