Interferometric Phase Microscopy for Label-Free Morphological Evaluation of Sperm Cells  

Oleh:

Haifler, Miki ; Girshovitz, Pinhas ; Band, Gili ; Shaked, Natan T.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 104 no. 01 (Jul. 2015), page 43–47.
Topik: Interferometry; Phase Microscopy; Male Fertility; Sperm Analysis
Fulltext: F02 v104 n1 p43 kelik2016.pdf (633.09KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To compare label-free interferometric phase microscopy (IPM) to label-free and label-based bright-field microscopy (BFM) in evaluating sperm cell morphology. This comparison helps in evaluating the potential of IPM for clinical sperm analysis without staining. Design: Comparison of imaging modalities. Setting: University laboratory. Patient(s): Sperm samples were obtained from healthy sperm donors. Intervention(s): We evaluated 350 sperm cells, using portable IPM and BFM, according to World Health Organization (WHO) criteria. The parameters evaluated were length and width of the sperm head and midpiece; size and width of the acrosome; head, midpiece, and tail configuration; and general normality of the cell. Main Outcome Measure(s): Continuous variables were compared using the Student's t test. Categorical variables were compared with the ?2 test of independence. Sensitivity and specificity of IPM and label-free BFM were calculated and compared with label-based BFM. Result(s): No statistical differences were found between IPM and label-based BFM in the WHO criteria. In contrast, IPM measurements of head and midpiece width and acrosome area were different from those of label-free BFM. Sensitivity and specificity of IPM were higher than those of label-free BFM for the WHO criteria. Conclusion(s): Label-free IPM can identify sperm cell abnormalities, with an excellent correlation with label-based BFM, and with higher accuracy compared with label-free BFM. Further prospective clinical trials are required to enable IPM as part of clinical sperm selection procedures.

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