Clinical Significance of Intercellular Contact at the Four-Cell Stage of Human Embryos, and the Use of Abnormal Cleavage Patterns to Identify Embryos with Low Implantation Potential: A Time-Lapse Study  

Oleh:

Yanhe Liu ; Chapple, Vincent ; Feenan, Katie ; Roberts, Peter ; Matson, Phillip

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 103 no. 06 (Jun. 2015), page 1485–1491.
Topik: Embryo; Time Lapse; Intercellular Contact; In Vitro Fertilization; Abnormal Cleavage
Fulltext: F02 v103 n6 p1485 kelik2016.pdf (354.32KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective: To investigate the clinical significance of intercellular contact point (ICCP) in four-cell stage human embryos and the effectiveness of morphology and abnormal cleavage patterns in identifying embryos with low implantation potential. Design: Retrospective cohort study. Setting: Private IVF center. Patient(s): A total of 223 consecutive IVF and intracytoplasmic sperm injection treatment cycles, with all resulting embryos cultured in the Embryoscope, and a subset of 207 cycles analyzed for ICCP number where good-quality four-cell embryos were available on day 2 (n ¼ 373 IVF and n ¼ 392 intracytoplasmic sperm injection embryos). Intervention(s): None. Main Outcome Measure(s): Morphologic score on day 3, embryo morphokinetic parameters, incidence of abnormal biological events, and known implantation results. Result(s): Of 765 good-quality four-cell embryos, 89 (11.6%) failed to achieve six ICCPs; 166 of 765 (21.7%) initially had fewer than six ICCPs but were able to establish six ICCPs before subsequent division. Embryos with fewer than six ICCPs at the end of four-cell stage had a lower implantation rate (5.0% vs. 38.5%), with lower embryology performance in both conventional and morphokinetic assessments, compared with embryos achieving six ICCPs by the end of four-cell stage. Deselecting embryos with poor morphology, direct cleavage, reverse cleavage, and fewer than six ICCPs at the four-cell stage led to a significantly improved implantation rate (33.6% vs. 22.4%). Conclusion(s): Embryos with fewer than six ICCPs at the end of the four-cell stage show compromised subsequent development and reduced implantation potential. Deselection of embryos with poor morphology and abnormal cleavage revealed via time-lapse imaging could provide the basis of a qualitative algorithm for embryo selection. (Fertil Steril_ 2015;103:1485–91. _2015 by American Society for Reproductive Medicine.)

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali