Some epitopes conservation in non structural 3 protein dengue virus serotype 4  

Oleh:

Siregar, Tegar A. P ; Sudiro, T Mirawati ; Dewi, Beti Ernawati ; Lestari, Whinie

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Health Science Journal of Indonesia vol. 06 no. 02 (Dec. 2015), page 126-131.
Topik: dengue virus; NS3 protein; T cell epitope; B cell epitope

Ketersediaan

Perpustakaan PKPM Nomor Panggil: H25 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: H25.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Background: Non Structural 3 (NS3) protein of dengue virus (DENV) is known to induce antibody, CD4+ and CD8+ T cell responses, and playing role in viral replication. NS3 protein has T and B cell epitopes, which has conservation difference between DENV-4 strains. This study aimed to identify conservation of T and B cell epitope in NS3 protei among DEN4 strains and four serotypes DENV of Indonesia strains. Methods: Research was held at the Department of Microbiology, Faculty of Medicine, Universitas Indonesia, June 2013 to April 2014. NS3 amino acid sequenceof DENV-4 081 strain was obtained after NS3 gene of DENV-4 081 PCR products were sequenced. T and B cell epitopes of NS3 protein of DENV-4 081 strain were analised and compared to NS3 proteins of 124 DENV-4 strains around the world and four serotypes of Indonesia strains. World strains were isolated from America (i.e. Venezuela, Colombia, etc.) and Asia (i.e. China, Singapore, etc.). For the comparison, T and B cell epitope positions of NS3 protein were obtained from published report. Results: Eight positions of T cell epitopes and two positions of B cell epitopes of NS3 DENV-4 081 were identical and conserved to NS3 protein of 124 DENV-4 strains around the world. B cell epitope of NS3 DENV-4 081 protein at aa 537-544 was found identical and conservedto four serotypes DENV of Indonesia strains. Conclusion: This wide conservation of T and B epitopes in almost all DENV-4 strains around the world and all serotypes of Indonesia strains.

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