Neopterin dan Peroksida Serum sebagai Petanda Makrofag Teraktivitasi pada Tuberkulosis Paru Aktif dan Individu Berbahaya Tinggi  

Oleh:

Wande, I Nyoman ; Linawati, Ni Made ; Bagiada, I Made ; Yasa, IWP Sutirta ; Subawa, AAN

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 22 no. 01 (Nov. 2015), page 77-81.
Topik: Actived Macrophages; Serum Neoptrin; Serum Peroxide

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Failure of macrophages to phagocytize Mycobacterium tuberculosis causes the release of hydrogen peroxide/peroxide (H2O2) by the activated macrophages. Neopterin is one of the most important markers in the activated macrophages. Neopterin is a pteridine derivative produced by the activated macrophages through the stimulation of interferon gamma. Increased levels of Neopterin has been reported in lung tuberculosis. Activation of macrophages is essential to the development of tuberculosis infection that can lead to active pulmonary tuberculosis or latent tuberculosis, in this case is a high-risk for healthy individuals. To determine the differences in serum levels of Neopterin and H2O2 between patients with active pulmonary tuberculosis and healthy individuals at high risk of pulmonary tuberculosis. A total of 15 patients with active pulmonary tuberculosis and 15 healthy individuals at high risk examination serum Neopterin levels and peroxide (H2O2). Active pulmonary tuberculosis patients when the results of sputum smear examination chest x-rays is obtained positive results. Healthy individuals at high risk when the results of smear examination and chest x-rays is negative. The level of Neopterin was examined using a double antibody sandwich immunoassay with Human neopterin (NEOP) ELISA Kit ® Bioassay Technology Laboratory. The level of peroxide was examined using quantitative colorimetric peroxidedetermination with QuantiChromTM Peroxide Assay Kit (DIOX-250)®. Neopterin serum levels between patients with active pulmonary tuberculosis and healthy individuals at high-risk were analysed by independent samples t-test. H2O2 serum levels between patients with active pulmonary tuberculosis and healthy individuals at high-risk were analysed with Mann Whitney Test. The confidence level is p <0.05. The mean Neopterin levels in patients with active pulmonary tuberculosis was 5.17±4.64 nmol/L, the mean Neopterin levels in group of healthy individuals at high risk was 3.97±1.79 nmol/L. Statistical analysis by the independent samples t-test found no significant differences between groups in Neopterin serum levels of patients with active pulmonary tuberculosis and healthy individuals at high risk (p=0.357). The mean serum levels of H2O2 of group of patients with active pulmonary tuberculosis was 26.38±3.00 µM, the mean levels of H2O2of group of healthy individuals at high risk of 20.69±4.46 µM. Statistical analysis with non-parametric Mann-Whitney Test was found significant difference in the peroxide (H2O2) levels between groups of patients with active pulmonary tuberculosis and group of healthy individual at high-risk (p=0.000). The levels of Neopterin in patients with active pulmonary tuberculosis was not significantly higher compared to the healthy individuals of high risk. Levels of peroxide (H2O2) serum in patients with active pulmonary tuberculosis was significantly higher compared to the group of healthy individuals at high risk. This shows that there is increased activity of macrophages in patients with active pulmonary tuberculosis, but not effective in eliminating of Mycobacterium tuberculosis.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali