Enhancement of ß-Glucosidase Activity in Penicillium sp. by Random Mutation with Ultraviolet and Ethyl Methyl Sulfonate  

Oleh:

Syafriana, Vilya ; Nuswantara, Sukma ; Mangunwardoyo, Wibowo ; Lisdiyanti, Puspita

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI - non-atma jaya
Dalam koleksi: Annales Bogorienses vol. 18 no. 02 (Dec. 2014), page 27-33.
Topik: ß-glucosidase; UV; EMS; Penicillium
Fulltext: AA772718022014.pdf (439.87KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: AA77 Non-tandon: tidak ada Tandon: 1

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The genus Penicillium has a potential ability to produce ß-glucosidase. The aim of the study was to improve the ß-glucosidase activity of Penicillium sp. ID10-T065 with physical (Ultraviolet = UV), chemical (Ethyl Methyl Sulfonate = EMS), and combined mutation (UV-EMS). The spores of Penicillium sp. ID10-T065 were exposed into UV irradiation for 3 minutes with dose of 0.1 J/cm 2 and 13 cm of distances. Chemical mutation was done by treated spores into 3% of EMS solution for an hour. Combined mutation of UV and EMS were also performed by UV for 3 minutes (0.1 J/cm 2 , 15 cm) and continued with soaking into 2-3% of EMS solution. The developed mutants were screened, selected and assayed. Comparison of enzyme activities with the wild- type (1.78 U/ml), mutant UV13 (5.53 U/ml) showed a 3.1 fold increase; mutant EM31 (4.26 U/ml) showed a 2.4 fold increase. Meanwhile, mutant UM23 obtained from the multiple exposures showed a decreased activity (1.75 U/ml). Mutant UV13 showed the best enzyme activity to be considered as a potential strain for ß-glucosidase producer. This result needs to be further elaborated especially on its genetic stability studies in order for the ascertained as a stable mutant.

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