High expression of survivin and its splice variants survivin ?Ex3 and survivin 2 B in oral cancers  

Oleh:

Mishra, Rupa ; Palve, Vinayak ; Kannan, Sadhana ; Pawar, Sagar ; Teni, Tanuja

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology (keterangan: ada di ClinicalKey) vol. 120 no. 04 (Oct. 2015), page 497–507 .

Ketersediaan

Perpustakaan FK Nomor Panggil: O04.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objectives We have previously reported inactivation of p53 in 46% of Indian patients with oral cancer. Survivin, a p53 target gene and an inhibitor of apoptosis protein (IAP), is overexpressed in several cancers, including oral cancers. Studies assessing the role of survivin and its splice variants in oral cancers are, however, rare. Materials and Methods The expression of 6 survivin isoforms in 4 oral cancer cell lines (AW8507, AW13516, UPCI-SCC040, UPCI-SCC029 B), a dysplastic oral cell line (DOK), 75 paired oral tumor and adjacent normal tissues, and 12 normal oral tissue samples from healthy individuals was analyzed by real-time PCR. The expression was correlated with clinicopathologic parameters, which included age, sex, tumor-node-metastasis (TNM) staging, tobacco and/or alcohol consumption, site, and differentiation status of tumor. Results This is the first study to find overexpression of the 6 characterized survivin isoforms in oral cancers compared with normal tissues (P < .05). Additionally, a significant (P < .05) correlation among the fold changes of all 6 survivin isoforms was observed. Survivin wild type (wt) was the predominantly expressed isoform in oral cell lines and tumor tissues versus normal tissues (P < .05). Among the minor isoforms, survivin ?Ex3 and survivin 2 B were dominantly expressed, whereas survivin 2 a and survivin 3 a overexpression was found for the first time. Further high survivin 3 B expression exhibited a significant association (P < .05) with poorly differentiated tumors. Interestingly the combined expression of the antiapoptotic survivin isoforms, survivin wt, survivin ?Ex3, and survivin 3 B, exhibited a significant association with TNM staging of the tumor. Conclusions Our studies thus indicate that oral cancers overexpress the antiapoptotic survivin variants, which exhibit an association with advanced tumor stage, implying a role for these variants in oral tumorigenesis.

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