SHORT COMMUNICATION : Role of Chloramphenicol Acetyltransferase (CAT) Enzyme for Early Detection of Chloramphenicol Resistant Salmonella typhi  

Oleh:

Nurtjahyani, Supiana Dian

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 08 no. 2 (Jun. 2014), page 81-85.
Topik: CATenzyme; chloramphenicol; resistant; Salmonella typhi
Fulltext: 299-828-1-PB_Ros.pdf (175.34KB)

Isi artikel

Microbial resistance to antibiotic is a major problem in the treatment of infectious diseases. The purpose of this study was to identification the role of CAT enzyme for early detection of chloramphenicol resistant Salmonella typhi . Observation was performed on Salmonella typhi isolated from Dr. Sutomo Hospital, Surabaya Health Laboratory, and Microbiology Laboratory-Airlangga University, Surabaya in 2009 and had been subcultured. The sub-culture was then tested for its susceptibility to chloramphenicol by using anti-chloramphenicol acetyl transferase (CAT) antibody from Sigma (catalog number C.9336). Susceptibility test using dilution and diffusion methods proved that resistant could change to sensitive and vice versa. It seems that the CAT enzyme can bind anti-CAT so reversing the resistant into sensitive and conversely, sensitive into resistant one.

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