The Synthesis of Polymeric Dual-Functional Antimicrobial Surface Based on Poly(2-methyl-2-oxazoline)  

Oleh:

Pidhatika, Bidhari ; Rakhmatullina, Ekaterina

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 19 no. 01 (2014), page 12-22.
Topik: biopassive; bioactive; PLL-g-PMOXA-QAC; dual-functional; click reaction
Fulltext: 266-707-1-PB_Ros.pdf (435.45KB)

Isi artikel

There is a high interest in the development of antimicrobial coatings to fi ght bacterial infections. We present the development of dual-functional antimicrobial surface, in which a biopassive platform was functionalized with bioactive compounds on the surface, using a graft copolymer system poly(L-lysine)-graftpoly( 2-methyl-2-oxazoline)-quarternery ammonium compound (PLL-g-PMOXA-QAC). Alkyne functionality was introduced to the PMOXA chain at a-terminus by initiating the living cationic polymerization of 2-methyl- 2-oxazoline with a propargylic-initiator. The reaction was terminated with carboxy derivative-terminator that allows grafting of the polymeric chain from the ß-terminus to poly(L-lysine) (PLL) backbone, resulting in graft copolymer alkynyl PLL-g-PMOXA. The conjugation between alkynyl PLL-g-PMOXA and QAC was then performed using click reaction. The chemical structures of the polymers were characterized by MALDI-TOF spectrometry and NMR spectroscopy. The results demonstrate that we have successfully synthesized PLL-g- PMOXA-QAC copolymer with grafting density (number of lysine/number of PMOXA) of 0.33. The resulting PLL-g-PMOXA-QAC copolymer was then immobilized onto carboxylated tissue cultured polystyrene (TCPS) surface and exposed to bacteria solution to test its dual-functional properties. Preliminary live-and-dead bacteria study indicates dual-functionality of the PLL-g-PMOXA-QAC-coated surface.

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