Comparative Analysis of Genes Induced by Respiratory Syncytial Virus and DsRNA in Human Epithelial Cells  

Oleh:

Limmon, Gino Valentino

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 18 no. 02 (2013), page 101-115.
Topik: RSV; dsRNA; transcriptome; immune response; infl ammation
Fulltext: 245-643-1-PB_Ros.pdf (468.24KB)

Isi artikel

Epithelial cells are the primary target of respiratory viral infections and play a pivotal role in virusinduced lung infl ammation and in anti viral immune response. A common signal for the presence of viral infections and induction of infl ammation is recognition of double stranded RNA (dsRNA). Thus far, there has not been a high-throughput transcrptome analysis of RSV- or dsRNA-induced genes in primary human bronchial epithelial cells (PHBE), nor there has been a comparison between dsRNA- and RSV-induced genes. To establish the transcriptome profi les and to determine the contribution of dsRNA in the induction of infl ammation during respiratory virus infection, we compared the gene expression profi les of PHBE cells that were infected with Respiratory Syncytial Virus (RSV) or were treated with dsRNA. Our transcriptome analysis showed that RSV infection and and dsRNA treatment induced up-regulation of 2024 and 159 genes in PHBE respectively. Comparison of genes revealed that RSV and dsRNA commonly induced 75 genes in PHBE cells. The common up-regulated genes were functionally grouped in multiple response pathways involved in infl ammation and immune responses. Interestingly, there were several previously unreported genes that were up-regulated in primary human epithelial cells that are relevant to a TH2 allergic phenotype. This comparison of a high-throughput gene expression study offers a comprehensive view of transcriptional changes induced by dsRNA and RSV, and importantly compares dsRNA-induced genes with RSV-induced genes in PHBE cells.

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