G protein–coupled estrogen receptor 1 agonist G-1 induces cell cycle arrest in the mitotic phase, leading to apoptosis in endometriosis  

Oleh:

Mori, Taisuke ; Ito, Fumitake ; Matsushima, Hiroshi ; Takaoka, Osamu ; Tanaka, Yukiko

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 103 no. 05 (May 2015), page 1228–1235.
Topik: GPER; G-1; endometriosis; cell cycle arrest; apoptosis

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: tidak ada Tandon: 1

Lihat Detail Induk

Isi artikel

Objective To demonstrate the effects of the selective G protein–coupled estrogen receptor 1 (GPER) agonist G-1 in human ovarian endometriotic stromal cells (ESCs). Design Experimental in vitro study. Setting University hospital. Patient(s) A total of 33 patients with ovarian endometrioma. Intervention(s) Endometriotic stromal cells from ovarian chocolate cysts were treated with the GPER agonist G-1. Main Outcome Measure(s) The primary outcomes were cell proliferation, measured using the WST-8 assay; cell cycle, as analyzed using flow cytometry, fluorescent immunocytochemistry, and cytotoxicity; caspase activity, as measured by fluorescent and luminescent enzyme assays; and protein expression levels, as determined by Western blot analysis. Result(s) G-1 suppressed ESC proliferation in a concentration-dependent manner. The inhibitory effect was not blocked when GPER signaling pathways, including the GPER itself, were inhibited. G-1 induced cell cycle arrest and accumulation in the sub-G1 phase in ESCs. Immunofluorescence analysis demonstrated that G-1 interrupted microtubule assembly at the mitotic phase. G-1 also induced caspase-3–dependent apoptosis without significant cytotoxicity. Conclusion(s) G-1 suppressed proliferation and induced apoptosis in ESCs, suggesting the potential use of this compound as a therapeutic drug for the treatment of endometriosis.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali