In vitro activity of terpenes against Candida albicans and ultrastructural alterations  

Oleh:

Martínez, Alejandra ; Rojas, Ninon ; Garcia, Loreto ; Gonzalez, Felipe ; Dominguez, Mariana ; Catalan, Alfonso

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology (keterangan: ada di ClinicalKey) vol. 118 no. 05 (Nov. 2014), page 553–559 .

Ketersediaan

Perpustakaan FK Nomor Panggil: O04.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective The purpose of this study was to investigate the in vitro activity of terpene blends combined with tissue conditioner against Candida albicans and the effect on its morphology and sub-micro structure. Study Design The minimal inhibitory concentration (MIC) of terpenes, obtained from a by-product of kraft pulping, was determined using broth microdilution against C. albicans strains, and the activity of terpenes combined with Coe-Comfort tissue conditioner was assessed. Cell morphologic alterations were evaluated using scanning electronic microscopy and transmission electronic microscopy. Data was analyzed using Student’s t test P < .05. Results The MIC of terpene blends fluctuated between 0.097% and 0.39% (v/v). Coe-Comfort tissue conditioner mixed with terpenes exhibited a total inhibition of C. albicans (P < .05). Terpenes induced ultrastructural alterations, even at the MIC value, including an increase in size, shape modification, cell wall damage with perforations, pronounced disconnection between cell wall and cytoplasm, and cytoplasmic vacuoles. Conclusions Terpenes had pronounced effects against C. albicans alone and in combination with Coe-Comfort tissue conditioner, which mainly resulted in cell wall damage.

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