Karakterisasi a-Amilase Bacillus firmus KH.9.4 Alkalotoleran dan Limbah Cair Tapioka  

Oleh:

Iswati, Ruma ; Tedja-Imas ; Nisa, Rachmania

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Biota Jurnal Ilmiah Ilmu-ilmu Hayati vol. IX no. 3 (Oct. 2004), page 129-135.
Topik: a-amylase activity; Bacillus firmus; tapioka liquid waste
Fulltext: BB74.1 129.pdf (225.35KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: BB74.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Alkalotolerant bacteria identified as Bacillus firmus KH.9.4 was isolated from tapioca liquid waste from Kedung Halang, Bogor. The isolate showed optimum a-amylase activity after 15 hours of cultivation on liquid media. Optimum enzyme activity occured at pH 6.0 and temperature 80'C. Upon heat treatment at pH 6.0 and 80'C the enzyme was still 100% active after 6 hours incubation without substrate. The enzyme activity was enhanced by supplement 10 mM MgSO4.7H2O. The presence of 1,5, and 10 mM ethylenediaminetertraacetic acid demonstrated the inhibitory effect to this enzyme. Zymogram analysis revealed one translucent zone corresponding to soluble starch activities after 60 minutes of incubation at 40'C in the 0.05 M phoshate buffer pH 7.0. Whereas SDS-PAGE analysis showed that there are 5 bands of proteins.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali