Profile of PRMT-1 Gene Polymorphism in Hemodialysis Patients with Increased ADMA Levels  

Oleh:

Thaha, Mochammad ; Nilamsari, Wenny P. ; Amin, M.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 46 no. 02 (Apr. 2014), page 97-103.
Topik: ADMA; PRMT-1 polymorphism; chronic kidney disease
Fulltext: hal 97 Profile of PRMT-1 Gene Polymorphism in Hemodialysis.pdf (1.6MB)

Ketersediaan

Perpustakaan FK Nomor Panggil: A02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Aim: to determine the distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis patients. Methods: genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection of 5 mL fasting venous blood. The blood was treated with phenol-chloroform extraction of genomic DNA. The candidate’s single nucleotide polymorphisms (SNPs) were genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all biochemical indicators of serum were examined. Results: fifty-seven hemodialysis patients participated in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism at sequence 5837 as there were different genotypes between C and G. Conclusion: the increased levels of ADMA might be caused by PRMT-1 gene polymorphism

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