Advanced glycation end products and their receptor contribute to ovarian ageing  

Oleh:

Stensen, Mette Haug ; Tanbo, Tom ; Storeng, Ritsa ; Fedorcsak, Peter

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 29 no. 01 (Jan. 2014), page 125-134.
Topik: female infertility; assisted reproduction; cell culture; cell signalling; advanced glycation end products

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K.2014.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

STUDY QUESTION Do advanced glycation end products (AGE) and the receptor for advanced glycation end products (RAGE) affect the cells of the human ovarian follicle? SUMMARY ANSWER AGE accumulate on the surface of ovarian granulosa–lutein (GL) cells and monocytes by binding to RAGE and other receptors with possible functional effects on these cells. WHAT IS KNOWN ALREADY AGE and RAGE are expressed in granulosa and theca cells, as well as in luteinized cells derived from the ovary. STUDY DESIGN, SIZE, DURATION In this prospective cohort study, human follicle fluid-derived cells were isolated from aspirates of ovarian follicles of women who underwent assisted reproduction treatment. PARTICIPANTS/MATERIALS, SETTING, METHODS Immunofluorescence microscopy and multi-colour flow cytometry were used to determine the presence of AGE and RAGE on the surface of follicular fluid-derived cells and to characterize downstream effects of RAGE activation. MAIN RESULTS AND THE ROLE OF CHANCE GL cells and ovarian monocytes were found to contain AGE and RAGE and to bind AGE–bovine serum albumin (BSA) in correlation with the patients' chronological age. AGE–BSA and BSA failed to induce significantly the cleavage of caspase-3, phosphorylation of nuclear factor-?B or the binding of annexin V (the latter was marginally increased). AGE-fibronectin was found to induce detachment of cultured GL cells in vitro. LIMITATIONS, REASONS FOR CAUTION The impact of AGE and RAGE in the ovary, shown here in cells in culture, remains to be affirmed in clinical settings. WIDER IMPLICATIONS OF THE FINDINGS The ligands of RAGE and their effects in the ovary remain uncertain but this study implies that AGEs in the form of structural long-lived extracellular matrix proteins, rather than soluble AGEs, may play a role in the decline of ovarian function during ageing.

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