High Frequency Spontaneous Deletions within the IcaADBC Operon of Clinical Staphylococcus epidermidis Isolates.  

Oleh:

Nuryastuti, Titik ; van der Mei, Henny C. ; Busscher, Henk J. ; Kuijer, Roel ; Aman, Abu Tholib ; Krom, Bastiaan P.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 17 no. 2 (Dec. 2012), page 144-153.
Topik: deletion; ica genes; Staphylococcus epidermidis; IcaADBC operon
Fulltext: 142_Ros.pdf (201.94KB)

Isi artikel

Staphylococcus epidermidis has been shown to undergo a phase variation correlating with expression of the icaADBC operon which contributes to biofi lm formation. Biofi lm formation of Enterococcus faecalis is related to heterogeneity in electrophoretic mobility. Here the relationship between phase variants of clinical isolates of S. epidermidis, icaADBC presence and electrophoretic mobility distributions is investigated. Of 105 S. epidermidis clinical isolates, 5 showed phase variation on Congo Red agar plate. Biofi lm forming capability of the black colonies and inability of the red colonies were confi rmed using a microtiter plate assay and confocal laser scanning microscopy. Upon analysis of electrophoretic mobility distributions, the black colonies displayed heterogeneity at pH 2 which was absent in the red colonies of the same strain. Surprisingly, it was shown that in all red colonies had lost the icaADBC genes. Determination of gene copy number using Real Time PCR targeting icaA showed reduction of gene copy within a culture with phase variation. In conclusion, using three fundamentally different approaches phase variation of the fi ve clinical isolates was observed. Variants appeared through loss of icaA and icaC gens. To our knowledge this is the fi rst report indicating S. epidermidis strains irreversible switching from biofi lm + to biofi lm – phenotype by deletion of ica genes.

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