Genetic Variation Analysis of Mold (Magnaporthe oryzae B.Couch) Using Random Amplifi ed Polymorphic DNA  

Oleh:

Pramono, Ajeng Kusumaningtyas ; Daryono, Budi Setiadi

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 17 no. 2 (Dec. 2012), page 90-95.
Topik: Magnaporthe oryzae; RAPD; mating type
Fulltext: 137_Ros.pdf (776.89KB)

Isi artikel

Magnaporthe oryzae B.Couch is a host-specifi c fungi, certain strain only infect certain host plant species. Genetic variety among M. oryzae isolates was explained by dendogram which was constructed using similarity data of Random Amplifi ed Polymorphic DNA (RAPD). Dendogram construction was achieved by computer software, Numerical Taxonomy System (NTSYS). The aim of the research were to study the genetic variation among M. Oryzae using RAPD and to construct a dendogram of genetic similarities among the ten isolates from green foxtail (Setaria viridis L.), fi nger millet (Eleusine coracana L.) and rice (Oryza sativa L.).RAPD was performed in 30 cycles using 5 primers (OPA-02, OPA-03, OPA-04, OPA-05, OPA-07). Polymorphism data was used to constructed dendogram using Dice index and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) in NTSYS software. There were 68 polymorphism fragments from 74 amplifi ed fragments. Three clusters were formed in the dendrogram, based on host pathotype: foxtail millet type, fi nger millet type and rice type. There were two subclusters in foxtail millet type based on mating type, MAT1-1 dan MAT1-2. Thus, RAPD could be used as a method for genetic variation analysis of Magnaporthe oryzae to show hostspecifi c specifi city.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali