Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT  

Oleh:

Lalitkumar, Sujata ; Boggavarapu, Nageswara R. ; Menezes, Judith ; Dimitriadis, Evdokia ; Zhang, Jian-Guo

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 100 no. 04 (Oct. 2013), page 1160-1169.
Topik: AKT; apoptosis; embryo attachment; LIF; PEGLA

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To study the effect of polyethylene glycated leukemia inhibitory factor (LIF) antagonist (PEGLA) in the human blastocyst viability and implantation process. Design In vitro study. Setting University hospital and research laboratory. Patient(s) Endometrial biopsy samples from fertile donors (n = 20), and surplus, frozen, good-quality human embryos obtained from an in vitro fertilization (IVF) clinic that survived thawing (n = 51). Intervention(s) Timed human endometrial biopsy on the day of luteinizing hormone peak + 4 days (LH + 4). Main Outcome Measure(s) Human embryo attachment rate, embryo quality, and expression of AKT and caspase-3. Result(s) PEGLA significantly reduced the embryo attachment rate to the endometrial construct. It decreased both mRNA and protein for LIF in the endometrial construct. Inhibition of embryonic LIF triggered apoptosis. Analysis of these blastocysts by immunofluorescence and real-time polymerase chain reaction showed a down-regulation in AKT activation and an increase in caspase-3 activation compared with the control group of blastocysts. Conclusion(s) The LIF inhibitor PEGLA could be a potential nonsteroidal fertility-regulating agent in humans. It acts on endometrial epithelial cells by down-regulating endometrial epithelial LIF. Inhibition of blastocyst LIF decreased its cell survival factor p-AKT and increased apoptosis (cleaved caspase-3). This highlights that embryonic LIF is vital for human embryo implantation.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali