Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells  

Oleh:

Chen, Ming-hui ; Li, Tao ; Ding, Chen-Hui ; Xu, Yan-wen ; Guo, Lu-yan

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 100 no. 05 (Nov. 2013), page 1444-1450.
Topik: Gene expression; growth differential factor-9; hormone; theca

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To explore the effect of growth differential factor-9 (GDF-9) alone on cell proliferation, cell viability, steroidogenesis, and hormone-stimulated gene expression in cultured mouse theca interstitial cells. Design Basic research. Setting University hospital. Animal(s) Immature 3- to 4-week-old SPF KM mice obtained from the Laboratory Animal Center of Sun Yat-Sen University. Intervention(s) Addition of GDF-9 at different dosages to primary culture of mouse theca interstitial cells. Main Outcome Measure(s) Cell number, cell viability, progesterone and testosterone levels, and hormone-stimulated gene mRNA abundance. Result(s) Growth differential factor-9 mildly increased the number of mouse theca interstitial cells and cell viability in a dose-dependent manner and mildly inhibited the production of progesterone in mouse theca interstitial cells. Administration of GDF-9 at the dosages of 200 ng/mL and 400 ng/mL resulted in a significant decrease in the testosterone level compared with the control group by 60.42% and 68.76%, respectively. Growth differential factor-9 significantly suppressed Lhcgr mRNA by 47.36%, Cyp11a1 mRNA by 62.30%, and Cyp17a1 mRNA by 55.39%, but had only a mild effect on Star gene expression. Conclusion(s) Growth differential factor-9 can inhibit the production of testosterone in mouse theca interstitial cells and suppress the corresponding gene expression.

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