Evaluation of cryopreserved ovarian tissue from prepubertal patients after long-term xenografting and exogenous stimulation  

Oleh:

Luyckx, Valerie ; Scalercio, Sarah ; Jadoul, Pascale ; Amorim, Christiani Andrade ; Soares, Michelle

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 100 no. 05 (Nov. 2013), page 1350-1357.
Topik: Fertility preservation; prepubertal patients; cryopreserved ovarian tissue; ovarian tissue transplantation; xenografting

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To assess follicular development after long-term xenotransplantation and exogenous stimulation of cryopreserved prepubertal ovarian tissue. Design Pilot study. Setting Gynecology research unit in a university hospital. Patient(s) Cryopreserved ovarian fragments were obtained from five prepubertal patients aged 7.2–12.2 years. Intervention(s) Xenografting of frozen-thawed prepubertal ovarian fragments to SCID mice for 5 months and exogenous stimulation. Main Outcome Measure(s) Follicular density, morphology, proliferation, development. Result(s) Follicular density varied between 1.05 and 47.89 follicles/mm2 in frozen-thawed ovarian tissue and 0.48 and 32.74 follicles/mm2 in grafted prepubertal ovarian tissue. Growing follicles at the last stage of follicular development were observed at significantly higher proportions after grafting. No statistical difference was evidenced in the number of primordial follicles, representing the largest proportion of the follicle pool (99.51% before grafting and 92.46% after grafting). Ki67 and antimüllerian hormone expression were observed in these growing follicles. Conclusion(s) This is the first description of transplantation of human cryopreserved prepubertal ovarian tissue to mice, demonstrating that a very high number of follicles survive after transplantation and a large pool of primordial follicles remains dormant. Growing follicles were observed, proving the responsiveness of prepubertal ovarian tissue to gonadotropins.

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