Estrogen receptor ß agonist diarylpropionitrile inhibits lipopolysaccharide-induced regulated on activation normal T cell expressed and secreted (RANTES) production in macrophages by repressing nuclear factor ?B activation  

Oleh:

Huang, Shi-ying ; Xin, Hong ; Sun, Jing ; Li, Rui ; Zhang, Xue-mei

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 100 no. 01 (Jul. 2013), page 234-240.
Topik: DPN; estrogen receptor beta; macrophage; RANTES; NF-?B

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To investigate the effect of the estrogen receptor-ß (ERß) agonist diarylpropionitrile (DPN) on lipopolysaccharide (LPS)-induced regulated on activation normal T cell expressed and secreted (RANTES) production in macrophages and the possible mechanisms. Design Cellular and molecular biology experimental study. Setting University-based research laboratory. Patient(s) None. Intervention(s) ERß mRNA and protein expression determined in murine macrophage cell line RAW264.7 cells using reverse-transcription polymerase chain reaction and Western blot analysis; RANTES production detected by ELISA in LPS-stimulated RAW264.7 cells and ERß knockdown RAW264.7 cells after the addition of DPN, phosphorylation of p65 and I?B degradation detected by Western blot analysis; and nuclear accumulation of p65 visualized using immunofluorescence. Main Outcome Measure(s) LPS-induced RANTES production and phosphorylation of p65 and I?B. Result(s) ERß was expressed in RAW264.7 cells, and DPN statistically significantly decreased LPS-induced RANTES production in RAW264.7 cells. Small interfering RNA targeting the ERß gene inhibited the effect of DPN on RANTES production. In addition, DPN inhibited nuclear translocation and phosphorylation of p65 by inhibiting I?B degradation and thus prohibited the activation of nuclear factor ?B (NF-?B). Conclusion(s) Diarylpropionitrile down-regulates LPS-induced RANTES production via ERß. This effect of DPN is likely due to repression of nuclear factor ?B activation.

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