Detection of Attaching and Effacing Virulence Gene of e.Coli  

Oleh:

Soleha, Maratu

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Health Science Journal of Indonesia vol. 04 no. 01 (Jun. 2013), page 41-46.
Topik: E.coli Virulence Genes; Real-Time PCR; Environmental Water; Perairan Lingkungan
Fulltext: H25 v4(1) jun13 p41,win.pdf (148.19KB)

Ketersediaan

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Isi artikel

Background: Escherichia coli (E.coli) bacteria have developed into pathogenic bacteria that caused diseases such as hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), sepsis, pneumonia, neonatal meningitis, and urinary tract infections. Pathogenic E. coli have acquired pathogenic/virulence genes from other bacteria in their environment. E.coli that normally lived in the human gut had adapted to other niches such as soil, food and the urinary tract. This study investigated the presence of pathogenic E.coli from water samples by examining E.coli virulence genes present in E.coli genomes of water sourced isolates. Methods: This study used Real-time PCR to detect virulent E.coli and sequencing which was then matched to virulent E. coli listed in BLAST as a reference. Results: E.coli sequence from RT PCR was matched with eae DNA segment pattern in BLAST. E.coli isolates tested with eae gene were detected in 7.3% of E.coli strains isolated from environmental water sources. Conclusion: The presence of virulent E.coli strains was detected in environmental water sources using Real time PCR.

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