CD36 and SR-BI Are Involved in Cellular Uptake of Provitamin A Carotenoids by Caco-2 and HEK Cells, and Some of Their Genetic Variants Are Associated with Plasma Concentrations of These Micronutrients in Humans  

Oleh:

Borel, Patrick ; Lietz, Georg ; Goncalves, Aurelie ; de Edelenyi, Fabien Szabo ; Lecompte, Sophie

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: JN: The Journal of Nutrition vol. 143 no. 04 (Apr. 2013), page 448-456.
Topik: Nutrient Physiology; Metabolism; Nutrient-Nutrient Interactions

Ketersediaan

Perpustakaan FK Nomor Panggil: J42.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Scavenger receptor class B type I (SR-BI) and cluster determinant 36 (CD36) have been involved in cellular uptake of some provitamin A carotenoids. However, data are incomplete (e.g., there are no data on a-carotene), and it is not known whether genetic variants in their encoding genes can affect provitamin A carotenoid status. The objectives were 1) to assess the involvement of these scavenger receptors in cellular uptake of the main provitamin A carotenoids (i.e., ß-carotene, a-carotene, and ß-cryptoxanthin) as well as that of preformed vitamin A (i.e., retinol) and 2) to investigate the contribution of genetic variations in genes encoding these proteins to interindividual variations in plasma concentrations of provitamin A carotenoids. The involvement of SR-BI and CD36 in carotenoids and retinol cellular uptake was investigated in Caco-2 and human embryonic kidney (HEK) cell lines. The involvement of scavenger receptor class B type I (SCARB1) and CD36 genetic variants on plasma concentrations of provitamin A carotenoids was assessed by association studies in 3 independent populations. Cell experiments suggested the involvement of both proteins in cellular uptake of provitamin A carotenoids but not in that of retinol. Association studies showed that several plasma provitamin A carotenoid concentrations were significantly different (P < 0.0083) between participants who bore different genotypes at single nucleotide polymorphisms and haplotypes in CD36 and SCARB1. In conclusion, SR-BI and CD36 are involved in cellular uptake of provitamin A carotenoids, and genetic variations in their encoding genes may modulate plasma concentrations of provitamin A carotenoids at a population level. Footnotes ?1 The SUVIMAX study was funded by Direction Générale de la Santé (Ministry of Health) and supported by Médéric, Ipsen, Mutuelle Générale de l'Education Nationale, Sodexho, and Pierre Fabre. Work on the subset population of SUVIMAX was granted by Agence Nationale de la Recherche (no. ANR-05-PNRA-010). The HELENA Study was funded by the European Union’s Sixth RTD Framework Programme (grant no. FOOD-CT-2005-007034); Universidad Politécnica de Madrid (grant no. CH/018/2008); Axis-Shield Diagnostics, Ltd. (Oslo, Norway); Abbot Científica S.A. (Spain); the Spanish Ministry of Education (EX-2007-1124); and Cognis GmbH (Germany). The Fish Oil Intervention and Genotype (FINGEN) study was supported by contract no. RRD7/N02/A from the UK Food Standards Agency.

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