The Effects of Oral Plain Kefir Supplementation on Proinflammatory Cytokine Properties of the Hyperglycemia Wistar Rats Induced by Streptozotocin  

Oleh:

Hadisaputro, Suharyo ; Djokomoeljanto, R.R.J. ; Judiono ; Marsetyawan H.N.E.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 44 no. 2 (Apr. 2012), page 100-104.
Topik: Probiotic; plain kefir; diabetes mellitus; hyperglicemia; free radicals; proinflamatory cytokines
Fulltext: The Effects of Oral Plain Kefir Supplementation.pdf (1.11MB)

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Perpustakaan FK Nomor Panggil: A02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Aim: to validate the effect of plain kefir on immune responses of hyperglycemia wistar rats induced by Streptozotocin. Methods: the randomized pretest - posttest control group study design was conducted in male hyperglycemia Wistar rats induced by streptozotocin (STZ). Rats were randomized into four groups: (1) STZinduced group were given insulin treatment 0.76 UI/200 g bw, (2) STZ-induced group and treated with plain kefir 3.6 cc/200 g bw/day for 30 days, (3) STZ-induced group as control, (4) normal animal group as a negative control. Blood glucose was measured from whole blood that was taken 0.1 ml from retroorbitalis vein by microhematocrit on day 1 (pretest) and day 30 (post test) by enzymatic methods. Immune responses (cytokines IL1, IL6, IL10, TNFa) were measured by ELISA. Data were analyzed by one way Anova, Mann Whitney test and Duncan with significant level of p<0.05. Results: plain kefir supplementation 3.6 cc/day affect blood glucose, proinflamatory cytokines (IL1, IL6, TNFa) and antiinflamatory cytokine (IL10). Statistical analysis showed decrease of glucose -111.00 + 44.23 ml (p<0.001) and proinflamatory cytokines IL1 about -18.62 + 23.59 and IL6 -3.21 + 7.57 mU/mL (p<0.001), respectively compared to the control groups. TNFa decreased 1.65 + 4.62 mU/mL, but not significant (p>0.05), except for controls group. In addition, antiinflammatory (IL10) showed also increase about 15.11 + 2.16 (p<0.05), except for the control. Conclusion: plain kefir supplementation significantly decreased blood glucose, level of cytokines (IL1, IL6) and lowered TNFa level. On the contrary, the level of IL10 is increased compare to control groups.

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