Expression and Activation of Glucocorticoids Receptor and Expression of Heat Shock Protein-90 of Lymphocyte Cell in Idiopathic Nephrotic Syndrome Patient  

Oleh:

Khairiyadi ; Subandiyah, Krisni ; Fitri, Loeki Enggar

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 25 no. 03 (Dec. 2009), page 122-127.
Topik: GR expression; GR activation; Hsp-90 in cytoplasm; Hsp-90 in nucleus; SSNS; SRNS

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Glucocorticoid receptors (GR) have an important role in the resistance of steroid therapy. Previous studies showed significant difference activation and expression of GR between Steroid Resistant Nephrotic Syndrome (SRNS) and Steroid Sensitive Nephrotic Syndrome (SSNS), however the other studies showed contrastive result. Previous studies also revealed Hsp-90 had an important role in GR activation. The aim of this study was to determine the differences expression and activation of GR and also Hsp-90 expression in lymphocytes cytoplasm and nucleus between SRNS and SSNS patients. A Cross-sectional study was conducted to 40 children (20 SRNS and 20 SSNS patients). Double stainning immunocytochemistry of lymphocytes was performed. The GR expression and activation were determined by counting amount of lymphocytes that express GR and Hsp-90 among 200 under light microscope with 1000x magnification. The differences of activation and activation of GR and Hsp-90 in cytoplasm and nucleus were analyzed with independent t-test and the correlation was analyzed with Pearson correlation. The study results showed the GR expression and also the Hsp-90 in cytoplasm and nucleus in SRNS was lower than SSNS group (p<0.05). There was no significant difference of GR activation (p=0.34 7) and no correlation between GR and Hsp-90 in cytoplasm (r=0.124).

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