Sumbangan All-Trans Asam Retinoat (ATRA) Bagi Penyembuhan Periodontitis  

Oleh:

Praptiwi ; Muis, Siti Fatimah ; Hadisaputro, Soeharyo

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI - non-atma jaya
Dalam koleksi: Media Medika Indonesiana vol. 45 no. 03 (Sep. 2011), page 169-173.
Topik: ATRA supplementation; collagen degradation; periodontitis

Ketersediaan

Perpustakaan FK Nomor Panggil: M30.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

All-trans retinoic acid (ATRA) contribution to periodontitis healing Background: In inflammation and infection of periodontal ligament i.e periodontitis, collagen fiber is damaged. All-trans retinoic acid (ATRA), metabolite of vitamin A, has potency to depress damage in inflammation. Indonesia with high vitamin A deficiency, facing to delayed recovery of periodontitis. The disease is vastly suffered by common people in this country. The study aimed to know the collagen status of periodontal ligament suffering periodontitis treated with tetracycline and ATRA supplementation. Method: The experimental study using post test only design in 12 adult male Wistar rat was done in 7 days. The animal study were induced periodontitis through Porphyromonas gingivalis inoculation. Tetracycline 90 mg/kg was given, beside ATRA supplementation of 10 mg/kg and 5 mg/kg. Four experimental groups were chosen randomly. Group of periodontitis + tetracycline was the control of the study. The independent variables were ATRA supplementation of those high and low dose. The dependent variable was collagen status. Collagen status consist of degradation and synthesis of collagen, indicated by mRNA MMP-2 and mRNA P1CP respectively. They were measured by reversed transcriptase polymerizing chain reaction and thin layer chromatography. All-trans retinoic acid concentration was measured using high performance liquid chromatography. The data analyzed using t-test, Oneway ANOVA, post hoc duncan and least significant differences. Result: In group receiving 10 mg ATRA/kgBB, the ATRA level was highest but the collagen degradation was lower although not significant compared to the low dose ATRA group. In both groups the degradation was significantly lower than the control group (p=0.007). The synthesis of collagen in the low dose ATRA group was higher than the high dose ATRA group and the control group. Conclusion: High dose of ATRA supplementation contribution to less degradation of collagen, but in term of collagen synthesis, low dose ATRA supplementation gives better result.

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