Wharton’s Jelly–Derived Mesenchymal Stem Cells: Isolation and Characterization  

Oleh:

Antoninus, Andreas Ardhika ; Agustina, Dwi ; Wijaya, Laura ; Hariyanto, Vincent ; Yanti ; Merlina, Maurin ; Setiawan, Boenjamin ; Bachtiar, Indra

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Cermin Dunia Kedokteran vol. 39 no. 08/196 (Aug. 2012), page 588-591.
Topik: Wharton’s jelly-mesenchymal stem cell; fetal bovine serum; isolation; characterization
Fulltext: 09_196Whartons Jelly–Derived Mesenchymal Stem Cells-Isolation and Characterization.pdf (515.57KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: C04.K.2012 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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The Wharton’s jelly-derived mesenchymal stem cell (WJ-MSCs) from the umbilical cord has been shown to have faster proliferation rates and greater expansion capability compared with others adult MSCs. In this work, the isolation and culture of WJ-MSCs using fetal bovine serum (FBS) as supplement was investigated in term of growth kinetics, differentiation capacity, and cell surface markers. WJ-MSCs of 3 donors were culturedin MEM Alpha medium supplemented with 20% FBS. Growth kinetics, cell surface markers, and in vitro differentiation potential toward the adipogenic, chondrogenic and osteogenic lineage were investigated. The study demonstrated that WJ-MSCs can be isolated and cultured in MEMAlpha medium supplemented with 20% FBS. Differentiation capacity was preserved throughout long-term culture. Immunophenotype assay showed similar MSCs characteristics with the exception of a distinct CD14-positive side population. However the CD14-positive side population tends to diminish with prolonged culture. All these parameters matched quite well with MSCs characteristics. Thus we established an efficient protocol for propagation of human WJ-MSCs. protocol for propagation of human WJ-MSCs.

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