Potential of inner cell mass outgrowth and amino acid turnover as markers of quality in the in vitro fertilization laboratory  

Oleh:

Gada, Ravi P. ; Daftary, Gaurang S. ; Walker, David L. ; Lacey, Jean M.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 98 no. 04 (Oct. 2012), page 863-869.
Topik: IVF quality control; inner cell mass (ICM); murine embryo assay (MEA); formaldehyde

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2012.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To compare sensitivity of inner cell mass (ICM) outgrowth assay and analysis of culture media amino acid turnover with the sensitivity of the human sperm motility assay (HSMA) and murine embryo assay (MEA) for detection of formaldehyde toxicity. Design Prospective in vitro study. Setting University hospital–based infertility center. Animal(s) Murine embryos. Intervention(s) The HSMA, MEA, and ICM outgrowth assays were performed with media containing 0–64-µM concentrations of formaldehyde. These assays were compared with dynamics of amino acid turnover in culture media. Main Outcome Measure(s) The lowest concentration of formaldehyde in culture media detected by each quality control assay. Result(s) Sperm forward progression, but not motility, detected formaldehyde at a concentration of 32 µM. Sperm motility index identified formaldehyde toxicity at 64 µM, whereas blastocyst rates in the MEA were affected at 32 µM formaldehyde. Evaluation of ICM using outgrowth and grade detected 16 µM formaldehyde. Leucine turnover in culture media detected 64 µM formaldehyde in the amino acid assay. Conclusion(s) Inner cell mass outgrowth is a more sensitive bioassay than MEA and HSMA for the detection of formaldehyde in culture media. Amino acid metabolism may also provide a sensitive quality control measure for detection of formaldehyde.

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