Recording and sorting live human sperm undergoing acrosome reaction  

Oleh:

Zoppino, Felipe Carlos Martín ; Halon, Narciso D. ; Bustos, Matias A. ; Pavarotti, Martin A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 97 no. 06 (Jun. 2012), page 1309-1315.
Topik: ANDROLOGY; Acrosome reaction; human sperm; Pisum sativum agglutinin; flow cytometry; acrosomal matrix

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2012.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To develop and evaluate a method to detect acrosome reaction (AR) in live human sperm. Design Prospective study. Setting Basic research laboratory. Patient(s) Human semen samples with normal parameters obtained from healthy donors. Intervention(s) Acrosome reaction assays. Main Outome Measure(s) Fluorescence assessment of AR. Result(s) Evaluating acrosomal exocytosis in live human sperm is challenging. In this study, we report that in reacting sperm, Pisum sativum agglutinin conjugated to fluorescein isothiocyanate rapidly permeates into the acrosome when fusion pores open and stabilizes the acrosomal matrix, preventing the dispersal of the granule contents. Conclusion(s) Fluorescent Pisum sativum agglutinin can be used to visualize AR in real time, to determine the percentage of sperm undergoing exocytosis upon stimulation, and to separate the population of reacting sperm by flow cytometry.

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