Mercury (Hg)-Resistant Bacteria in Hg-Polluted Gold Mine Sites of Bandung, West Java Province, Indonesia  

Oleh:

Chaerun, Siti khoduah ; Hasni, Sakinah ; Wani, Edy San ; Moeis, Maelita Ramdani

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 6 no. 2 (Jun. 2012), page 57-68.
Topik: I6S rRNA gene sequences; in situ bioremediation. Mercury (Hg); phenotype; phylogenetic analysis
Fulltext: 166-616-2-PB_dv.pdf (1.68MB)

Isi artikel

In the present study, ten mercury-resistant heterotrophic bacterial strains were isolated from mercury- contaminated gold mine sites in Bandung, West Java Province, Indonesia. The bacteria (designated strains SKCSH1 - SKCSI110) were capable of growing well at ~2(X) ppm of 1 IgCl2 except for strain SKCSI18, which was able to grow at 550 ppm I IgCT3. The bacteria were mesophi lie and grew optimally at l%NaCl at neutral pi I with the optimal growthtemperature of 25-37°C. Phenotypiccharacterizationandphylogenetic analysis based on the 16SrRNA gene sequence indicated that the isolates were closely related to the family Xanthomonadaceae, Aeromonadaceae, and Pseudomonadaceae and they were identified as Pseudomonas spp.,Stenotropkomonas$p.t and Aeromonas sp. Eight bacterial strains were shown to belong to the Pseudomonas branch, one strain to the Stenotrophomonas branch and one strai n to the Aeromonas branch of the y-Proteobacteria. Phy logeny based on their 16SrRNAgenesequencesindicatedthatfouroftheisolatesfSKCSH 1,SKCSII4.SKCS117,SKCSI 19) could be classified as representatives of four novel species in the genus Pseudomonas that were allocated to /* moraviensis (96.96% similarity') and /'piecogossicida (94.53,96.61, and 96.73% similarity). Four other isolates could be allocated to /*. plecogossicida (97.57 and 98.66% similarity ) and /' hibiscico/a (99.97% similarity), one isolate to Stenotrophomonas africana (99.69% similarity ), and one other isolate to Aeromonas hydrophila subsp. ranae (99.43% similarity). The findings of this study provide the first information of the phylogenetically-diverse llg-resistant bacteria in the llg-polluted sites of Indonesia that may be highly useful for developing in situ bioremediationordetoxificationof I Ig-contaminated sitesin Indonesia.

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