Isolation and Identification of A Thermostable Amylase-Producing Bacterium from Hatuasa Hotspring  

Oleh:

Malle, Dominggus ; Picarima, Junus ; Huwae, Laury Chara ; Rahmawati, Indra ; Purbowasito, Wahyu

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 6 no. 2 (Jun. 2012), page 83-88.
Topik: Bacillus; hotsprings; isolation; thermostable amylase
Fulltext: 165-617-2-PB_dv.pdf (1.72MB)

Isi artikel

Hot springs are a common source of thermophyles which produce thermostable enzymes. The objective of this study was to isolate and identify thermostable amylase-producing bacteria from a local geothermal spring. An amylase–producing bacterium strain was isolated from this hot spring which excreted amylase after being grown on starch agar screening plates at 37 °C. It was identified as Bacillus megaterium using the method of 16S ribosomal DNA. The organism is a rod-shape and is a spore-forming bacterium. Maximum amylase production was achieved after incubation in the production media for 72 h. Preliminary analysis of the secreted amylase showed that the enzyme could bind to DEAE-Sepharose matrix and was discharged by eluting with 0.5 M NaCl. The partially purified enzyme was stable up to 75 °C, showing that this enzyme might have potential application in the starch-processing industry.

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