Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil  

Oleh:

Istianto, Yudhie ; Koesoemowidodo, Raden Setyo Adji ; Saputra, Harmoko ; Watanabe, Yoshio ; Pranamuda, Hardaning ; MARWOTO, BAMBANG

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 6 no. 1 (Mar. 2012), page 42-47.
Topik: Phenol Method; Rare-Actinomycetes; Selective Isolation Method; Soil Sample
Fulltext: 7.pdf (373.29KB)

Isi artikel

Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 µg mL-1) and nystatin (50 µg mL-1). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.

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